AgrobacTransV1, Main, Exploration, bibRecord, 000213

Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.

Identifieur interne : 000213 ( Main/Exploration ); précédent : 000212; suivant : 000214

Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.

Auteurs : Kyaw Aung [États-Unis] ; Xiufang Xin [États-Unis] ; Christy Mecey [États-Unis] ; Sheng Yang He [États-Unis]

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.

RBID : pubmed:27837488

Descripteurs français

English descriptors

KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Gene Expression (MeSH), Genes, Reporter (MeSH), Host-Pathogen Interactions (immunology), Intracellular Space (metabolism), Lycopersicon esculentum (immunology), Lycopersicon esculentum (microbiology), Microscopy, Confocal (MeSH), Plant Diseases (immunology), Plant Diseases (microbiology), Plants, Genetically Modified (MeSH), Protein Transport (MeSH), Pseudomonas syringae (genetics), Pseudomonas syringae (metabolism), Tobacco (microbiology), Transformation, Genetic (MeSH), Type III Secretion Systems (genetics), Type III Secretion Systems (metabolism).
MESH :
- chemical , genetics : Bacterial Proteins, Type III Secretion Systems.
- chemical , metabolism : Bacterial Proteins, Type III Secretion Systems.
- genetics : Pseudomonas syringae.
- immunology : Host-Pathogen Interactions, Lycopersicon esculentum, Plant Diseases.
- metabolism : Intracellular Space, Pseudomonas syringae.
- microbiology : Lycopersicon esculentum, Plant Diseases, Tobacco.
- Gene Expression, Genes, Reporter, Microscopy, Confocal, Plants, Genetically Modified, Protein Transport, Transformation, Genetic.

Abstract

Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector systems are a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors that target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular basis of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.

DOI: 10.1007/978-1-4939-6649-3_12
PubMed: 27837488
PubMed Central: PMC5643156

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000259
to stream Main, to step Curation: 000259

Le document en format XML

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<term>Bacterial Proteins (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Genes, Reporter (MeSH)</term>
<term>Host-Pathogen Interactions (immunology)</term>
<term>Intracellular Space (metabolism)</term>
<term>Lycopersicon esculentum (immunology)</term>
<term>Lycopersicon esculentum (microbiology)</term>
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<term>Plant Diseases (immunology)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Protein Transport (MeSH)</term>
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<term>Pseudomonas syringae (metabolism)</term>
<term>Tobacco (microbiology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Espace intracellulaire (métabolisme)</term>
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<term>Gènes rapporteurs (MeSH)</term>
<term>Interactions hôte-pathogène (immunologie)</term>
<term>Lycopersicon esculentum (immunologie)</term>
<term>Lycopersicon esculentum (microbiologie)</term>
<term>Maladies des plantes (immunologie)</term>
<term>Maladies des plantes (microbiologie)</term>
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<term>Protéines bactériennes (génétique)</term>
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<term>Végétaux génétiquement modifiés (MeSH)</term>
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<term>Lycopersicon esculentum</term>
<term>Maladies des plantes</term>
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<term>Gènes rapporteurs</term>
<term>Microscopie confocale</term>
<term>Transformation génétique</term>
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<front><div type="abstract" xml:lang="en">Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector systems are a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors that target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular basis of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.</div>
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<Abstract><AbstractText>Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector systems are a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors that target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular basis of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.</AbstractText>
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<ReferenceList><Reference><Citation>Genetics. 2015 May;200(1):47-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25745023</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2008 Feb;146(2):325-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18250230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biosci Bioeng. 2007 Jul;104(1):34-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17697981</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2007 Sep;51(6):1126-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17666025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2015 Sep;169(1):793-802</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26206852</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2009 Jul;59(1):169-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19309456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2014 Mar;201(4):1358-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24329768</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2013;1069:227-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23996319</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2005 Dec;2(12):905-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16299475</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2007;390:239-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17951692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2012 May;14(5):669-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22233353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2007 May 17;447(7142):284-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17450127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2007 Mar 20;17(6):499-508</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17350264</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2013 Apr;161(4):2049-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23396834</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2006 Feb;45(4):616-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16441352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10774-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21670267</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2010 Mar;12(3):318-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19863557</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2010;655:253-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20734266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1991 Jan;3(1):49-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1824334</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2015 Jun 10;17 (6):752-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26067603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 May;150(1):125-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19329564</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2014 Jan;77(2):310-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24299018</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
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Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.

Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.

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